Based on the range of replies I see here, I still can't determine if it will work or not. I wish it would, I'd like to put the drive I have to use, but certainly don't want to ruin it or lose any shows that would be recorded on it. Sucks.
If you're referring to using the brand new Seagate from the usb 3.0 enclosure in a new eSATA enclosure you won't lose anything. It's a new drive, nothing is on it.